Flow Cytometer


We know that people demand performance and reliability from their flow cytometers, even for standard applications. But uncompromised performance simply would not be achievable at a lower price point with conventional entry-level systems. So we designed our SE520EXi benchtop flow cytometer with the same patented technologies as our S1000 series—without the same price tag. Our SE520EXi may be a small flow cytometer, but it’s big on performance.



  • Fixed configuration with 2 lasers (488 nm and 640 nm) and 6 colors
  • Based on the same performance and patented technology as the S1000EXi
  • Complete compatibility with Stratedigm’s full automation suite



Technical Specifications


  • System is designed to support 2 lasers with spatial separation
  • Laser options:
    • Solid-state 488 nm – 150 mW
    • Solid-state 640 nm – 100 mW

Detector Parameters & Data Acquisition:

  • Forward scatter (FSC) – enables separation of unfixed platelets from noise
    • FSC resolution: < 500 nm polystyrene beads
    • FSC scales: log & linear
    • FSC: parameters: width, peak-height; area
    • Optional FSC photomultiplier (PMT) detector
  • Side scatter (SSC)- resolves lympho-, mono- , and granulocytes
    • SSC resolution: < 200 nm polystyrene beads
    • SSC scales: log & linear
    • SCC parameters: width, peak-height, area
  • Fluorescence channels:
    • Standard number of colors: 6
    • Sensitivity: < 100 MESF
    • PMT scales: log & linear
    • PMT parameters: width, peak-height, area
    • Time: 13 µsec resolution

Analysis Rate & Carryover

  • Analysis rate: 10,000 events/ sec
  • Carryover: < 0.1% automatic backlash between samples

Sample Input:

  • Dead volume: < 8 μL
  • Minimum sample volume: < 20 μL
  • Maximum particle size: 40 μm
  • Patented low insertion force single tube loading

Fluidics Tray:

  • Integrated fluidics tray does not increase the footprint of the instrument
  • Automated startup, shutdown, and cleaning cycles
  • Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
  • Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
  • Optional automated sheath tank fill and waste drainage

Operating Conditions:

  • Operating Conditions: 60-86 °F (15-30 °C)
  • Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
  • Weight (including fluidics tray): < 70 lbs (35 kg)
  • Power: 110/115/230 VAC, 50-60 Hz


U.S. Patent No. 7821631B1
U.S. Patent No. 8202059B1
U.S. Patent No. 8202733B1
U.S. Patent No. 8570500B2
U.S. Patent No. 9108196B1
U.S. Patent No. 9857284B1

Download Tech Specs


User Interchangeable Optical Filters:

Lasers# of ColorsColors/Filters
488 nm (Blue) Laser4FITC (530/30), PE (580/30), PerCP/PE-Cy5 (676/29), PE-Cy7 (740LP)
640 nm (Red) Laser2APC (676/29), APC-Cy7 (740LP)



To our friends and customers,

As we face the global challenge of COVID-19, please know that Stratedigm will continue to provide any support you may need so you can focus on work that makes a difference in these unprecedented times.

We are here to help our clients at the CDC, FDA, and other institutions involved in helping with the current crisis.

  • We have increased our remote service hours from 5 am to 8 pm PST, 7 days a week.
  • In order to ensure the safety of our staff and complying with the “stay at home” order currently in effect in our area, we switched all non-essential personnel to work remotely as required, while ensuring all customer support operations are functioning at an enhanced level.
  • You can reach our Sales and Customer Support staff by email, chat or phone (877-801-6098).
  • Manufacturing will continue to operate normally to ensure timely delivery of consumables and instrumentation. We continue to meet current demand and do not currently expect disruptions to product availability due to production shortages related to COVID-19. Stratedigm field service engineers will be available for onsite support, but may be impacted or otherwise delayed by travel restrictions.

We encourage you to stay creative and productive, keep your spirit strong, and most importantly - stay safe.

Team Stratedigm