SE520EON
Flow Cytometer
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We know that people demand performance and reliability from their flow cytometers, even for standard applications. But uncompromised performance simply would not be achievable at a lower price point with conventional entry-level systems. So we designed our SE520EON benchtop flow cytometer with the same patented technologies as our S1000 series—without the same price tag. Our SE520EON may be a small flow cytometer, but it’s big on performance.
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Features
- Fixed configuration with 2 lasers (488 nm and 640 nm) and 6 colors
- Based on the same performance and patented technology as the S1000EON
- Complete compatibility with Stratedigm’s full automation suite
Literature
Videos
Technical Specifications
Laser:
- System is designed to support 2 lasers with spatial separation
- Laser options:
- Solid-state 488 nm – 150 mW
- Solid-state 640 nm – 100 mW
Detector Parameters & Data Acquisition:
- Dynamic Range:
- 5 decades for acquisition
- 7 decades for analysis
- Forward scatter (FSC) – enables separation of unfixed platelets from noise
- FSC resolution: < 500 nm polystyrene beads
- FSC scales: log & linear
- FSC: parameters: width, peak-height; area
- Optional FSC photomultiplier (PMT) detector
- Side scatter (SSC)- resolves lympho-, mono- , and granulocytes
- SSC resolution: < 200 nm polystyrene beads
- SSC scales: log & linear
- SCC parameters: width, peak-height, area
- Fluorescence channels:
- Standard number of colors: 6
- Sensitivity: < 100 MESF
- PMT scales: log & linear
- PMT parameters: width, peak-height, area
- Time: 13 µsec resolution
Analysis Rate & Carryover
- Analysis rate: 20,000 events/ sec
- Carryover: < 0.1% automatic backlash between samples
Sample Input:
- Dead volume: < 8 μL
- Minimum sample volume: < 20 μL
- Maximum particle size: 40 μm
- Patented low insertion force single tube loading
Fluidics Tray:
- Integrated fluidics tray does not increase the footprint of the instrument
- Automated startup, shutdown, and cleaning cycles
- Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
- Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
- Optional automated sheath tank fill and waste drainage
Operating Conditions:
- Operating Conditions: 60-86 °F (15-30 °C)
- Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
- Weight (including fluidics tray): < 70 lbs (35 kg)
- Power: 110/115/230 VAC, 50-60 Hz
Patents:
U.S. Patent No. 7821631B1
U.S. Patent No. 8202059B1
U.S. Patent No. 8202733B1
U.S. Patent No. 8570500B2
U.S. Patent No. 9108196B1
U.S. Patent No. 9857284B1
Colors/Filters
User Interchangeable Optical Filters:
| Lasers | # of Colors | Colors/Filters |
|---|---|---|
| 488 nm (Blue) Laser | 4 | FITC (530/30), PE (580/30), PerCP/PE-Cy5 (676/29), PE-Cy7 (740LP) |
| 640 nm (Red) Laser | 2 | APC (676/29), APC-Cy7 (740LP) |