S1000EON
Benchtop Flow Cytometer
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Adding more colors and lasers to a newly designed cytometer is a logical but standard design improvement; Stratedigm is anything but standard.
Not only did we increase the number of parameters from 22 to 30 and the lasers from 4 to 6, we’ve also doubled our throughput, increased our dynamic range to 5 decades, created more spatially separated interrogation points, and boosted our sensitivity up to 300%! All of these major improvements are in the same footprint and at the same price as our first S1000 launched in 2008.
And with our Anti-Obsolescence Guarantee you’ll know your cytometer will be with you for many years to come. This is our commitment to Cytometry Without Compromise™.
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Features:
- Configurable and scalable up to 6 lasers and 30 parameters
- Best-in-class two-year warranty
- Future-proof system with easy field upgrades
- Excellent resolution < 2.5% CV
- Industry-leading sensitivity < 80 MESF
- Extensive range of particle sizes from 0.2 µm to 40 µm
- Patented 3rd Generation Smart Detect™ Technology
- Patented Laser-Trimming technology for simultaneous detection of particles from 0.2 µm to 7 µm
- Patented OptiSymmetric™ excitation architecture
- Revolutionary Ghost Detect™ module for measurement of background fluorescence without a physical trigger
- Seamless integration with automation options
Now available worldwide!
To configure your own flow cytometry instrument, automation platform, or learn more about our pricing, click the button below!
Literature
Technical Specifications
Laser:
- System is designed to support up to 6 lasers with spatial separation
- Laser options:
- Solid-state 372 nm – 100 mW
- Solid-state 405 nm – 100 mW
- Solid-state 488 nm – 150 mW
- Solid-state 532 nm – 100 mW
- Solid-state 552 nm – 100 mW
- Solid-state 561 nm – 100 mW
- Solid-state 640 nm – 100 mW
- Solid-state 805 nm – 100 mW
**Other lasers available upon request
Detector Parameters and Data Acquisition:
- Dynamic range:
- Acquisition: 5 decades
- Analysis: 7 decades
- Linearity: R2 > 99%
- Forward scatter (FSC) – enables separation of unfixed platelets from noise
- FSC resolution: < 500 nm polystyrene beads
- FSC scales: log and linear
- FSC parameters: width, peak-height, area
- Optional FSC photomultiplier (PMT) detector
- Optional Quad-FSC: 375, 405, 488, and/or 640 nm
- Side scatter (SSC) – resolves lympho-, mono-, and granulocytes
- SSC resolution: < 200 nm polystyrene beads
- SSC scales: log and linear
- SSC parameters: width, peak-height, area
- Optional Dual-SSC: 405 and/or 488 nm
- Fluorescence channels:
- Standard number of colors: 26
- Sensitivity: < 80 MESF
- PMT scales: log and linear
- PMT parameters: width, peak-height, area
- Time: 13 μsec resolution
Analysis Rate and Carryover:
- Analysis rate: up to 20,000 events/sec
- Carryover: < 0.1% with automatic backlash between samples
Sample Input:
- Dead volume: < 8 μL
- Minimum sample volume: < 20 μL
- Maximum particle size: 40 μm
- Patented low insertion force single tube loading
Fluidics Tray:
- Integrated fluidics tray does not increase the footprint of the flow cytomer
- Automated startup, shutdown, and cleaning cycles
- Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
- Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
- Optional automated sheath tank fill and waste drainage
Operating Conditions:
- Operating Conditions: 60-86 °F (15-30 °C)
- Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
- Weight (including fluidics tray): < 70 lbs (35 kg)
- Power: 110/115/230 VAC, 50-60 Hz
Patents:
U.S. Patent No. 7821631B1
U.S. Patent No. 8202059B1
U.S. Patent No. 8202733B1
U.S. Patent No. 8570500B2
U.S. Patent No. 9108196B1
U.S. Patent No. 9857284B1
U.S. Patent No. 9952136B2
Colors/Filters
User Interchangeable Optical Filters:
| Laser | Colors/Filters |
|---|---|
| 372 nm (Near-UV) Laser | BUV395 (405/20), DAPI (440/40), BUV496 (530/30), BUV563 (580/30), BUV661 (676/29), BUV737 (725/40), BUV805 (760LP) |
| 405 nm (Violet) Laser | BV421 (440/40), DAPI (440/40), BV480 (530/30), BV510 (520/40), BV570 (580/30), BV605 (615/30), BV650 (676/29), BV711 (725/40), BV750 (760LP), BV786 (760LP) |
| 488 nm (Blue) Laser | FITC (530/30), PE (580/30), PE-CF594 (615/30), PI (615/30), PE-Cy5 (676/29), PerCP (676/29), PE-Cy5.5 (725/40), PerCP-Cy5.5 (725/40), PE-Cy7 (760LP) |
| 552/561 nm (Yellow) Laser | PE (580/30), PE-CF594 (615/30), PI (615/30), mCherry (615/30), PE-Cy5 (676/29), PE-Cy5.5 (725/40), PE-Cy7 (760LP) |
| 640 nm (Red) Laser | APC (676/29), Alexa 700 (725/40), APC-R700 (725/40), APC-H7 (760LP) |
| 805 nm (IR) Laser | Alexa 790/BUV805 (810LP) |
**Other dyes available upon request
Performance
488 nm Laser
640 nm Laser
405 nm Laser
552 nm Laser
