S1000EON

Benchtop Flow Cytometer

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Adding more colors and lasers to a newly designed cytometer is a logical but standard design improvement; Stratedigm is anything but standard.

Not only did we increase the number of parameters from 22 to 30 and the lasers from 4 to 6, we’ve also doubled our throughput, increased our dynamic range to 5 decades, created more spatially separated interrogation points, and boosted our sensitivity up to 300%! All of these major improvements are in the same footprint and at the same price as our first S1000 launched in 2008. This is our commitment to Cytometry Without Compromise™.

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Features:

  • Configurable and scalable up to 6 lasers and 30 parameters
  • Best in class two-year warranty
  • Future-proof system with easy field upgrades
  • Excellent resolution < 2.5% CV
  • Industry-leading sensitivity < 80 MESF
  • Extensive range of particle sizes from 0.2 µm to 40 µm
  • Patented 3rd Generation Smart Detect™ Technology
  • Patented Laser-Trimming technology for simultaneous detection of particles from 0.2 µm to 7 µm
  • Patented OptiSymmetric™ excitation architecture
  • Revolutionary Ghost Detect™ module for measurement of background fluorescence without a physical trigger
  • Seamless integration with automation options

** Currently available in the United States; worldwide shipments coming soon.

Angled Right View of Stratedigm S1000EXi 4 Laser, 18 Color Flow Cytometer
Front View of Stratedigm S1000EXi 4 Laser, 18 Color Flow Cytometer
Right Angled View of Stratedigm S1000EXi 4 Laser, 18 Color Flow Cytometer
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Literature

Technical Specifications

Laser:

  • System is designed to support up to 6 lasers with spatial separation
  • Laser options:
    • Solid-state 372 nm – 100 mW
    • Solid-state 405 nm – 100 mW
    • Solid-state 488 nm – 150 mW
    • Solid-state 532 nm – 100 mW
    • Solid-state 552 nm – 100 mW
    • Solid-state 561 nm – 100 mW
    • Solid-state 640 nm – 100 mW
    • Solid-state 805 nm – 100 mW

**Other lasers available upon request

Detector Parameters and Data Acquisition:

  • Dynamic range:
    • Acquisition: 5 decades
    • Analysis: 7 decades
  • Forward scatter (FSC) – enables separation of unfixed platelets from noise
    • FSC resolution: < 500 nm polystyrene beads
    • FSC scales: log and linear
    • FSC parameters: width, peak-height, area
    • Optional FSC photomultiplier (PMT) detector
    • Optional Quad-FSC: 375, 405, 488, and/or 640 nm
  • Side scatter (SSC) – resolves lympho-, mono-, and granulocytes
    • SSC resolution: < 200 nm polystyrene beads
    • SSC scales: log and linear
    • SSC parameters: width, peak-height, area
    • Optional Dual-SSC: 405 and/or 488 nm
  • Fluorescence channels:
    • Standard number of colors: 26
    • Sensitivity: < 80 MESF
    • PMT scales: log and linear
    • PMT parameters: width, peak-height, area
    • Time: 13 μsec resolution

Analysis Rate and Carryover:

  • Analysis rate: up to 20,000 events/sec
  • Carryover: < 0.1% with automatic backlash between samples

Sample Input:

  • Dead volume: < 8 μL
  • Minimum sample volume: < 20 μL
  • Maximum particle size: 40 μm
  • Patented low insertion force single tube loading

Fluidics Tray:

  • Integrated fluidics tray does not increase the footprint of the instrument
  • Automated startup, shutdown, and cleaning cycles
  • Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
  • Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
  • Optional automated sheath tank fill and waste drainage

Operating Conditions:

  • Operating Conditions: 60-86 °F (15-30 °C)
  • Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
  • Weight (including fluidics tray): < 70 lbs (35 kg)
  • Power: 110/115/230 VAC, 50-60 Hz

 

Download Tech Specs

Colors/Filters

User Interchangeable Optical Filters:

LaserColors/Filters
372 nm (Near-UV) LaserBUV395 (405/20), DAPI (440/40), BUV496 (520/40), BUV563 (580/30), BUV661 (676/29), BUV737 (725/40), BUV805 (760LP)
405 nm (Violet) LaserBV421 (440/40), DAPI (440/40), BV480 (520/40), BV510 (520/40), BV570 (580/30), BV605 (615/30), BV650 (676/29), BV711 (725/40), BV750 (760LP), BV786 (760LP)
488 nm (Blue) LaserFITC (520/40), PE (580/30), PE-CF594 (615/30), PI (615/30), PE-Cy5 (676/29), PerCP (676/29), PE-Cy5.5 (725/40), PerCP-Cy5.5 (725/40), PE-Cy7 (760LP)
552/561 nm (Yellow) LaserPE (580/30), PE-CF594 (615/30), PI (615/30), mCherry (615/30), PE-Cy5 (676/29), PE-Cy5.5 (725/40), PE-Cy7 (760LP)
640 nm (Red) LaserAPC (676/29), Alexa 700 (725/40), APC-R700 (725/40), APC-H7 (760LP)
805 nm (IR) LaserAlexa 790/BUV805 (810LP)

**Other dyes available upon request

Performance

488 nm Laser

640 nm Laser

405 nm Laser

552 nm Laser

Videos

Brochure

 

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