S1000EXi

Flow Cytometer

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The idea behind the S1000 has never waivered: to design the ultimate flow cytometer. The best fluidics, fused with patented optical technology and the most powerful lasers—all within a benchtop package. And that commitment continues with our all-new 4 laser, 18 color analyzer. Like the S1000 predecessors, the S1000EXi  delivers an unrivaled flow experience. 

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Features:

  • Configurable and scalable up to 4 lasers and 18 colors
  • Best in class two-year warranty
  • Future-proof system with easy field upgrades
  • Excellent resolution < 2.5% CV
  • Industry-leading sensitivity < 100 MESF
  • Extensive range of particle sizes from 0.2µm to 40µm
  • Patent pending Laser-Trimming technology for detection of particles from 0.2 µm to 7µm simultaneously
  • Patented OptiSymmetric™ excitation architecture and Smart Detect™ technology
  • Revolutionary Ghost Detect™ module for measurement of background fluorescence without a physical trigger
  • Seamless integration with automation options
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Technical Specifications

Laser:

  • System is designed to support up to 4 lasers with spatial separation
  • Laser options:
    • Solid-state 405 nm – 100 mW
    • Solid-state 488 nm – 150 mW
    • Solid-state 532 nm – 100 mW
    • Solid-state 640 nm – 100 mW
  • Custom laser options:
    • Solid-state 372 nm – 100 mW
    • Solid-state 552 nm – 100 mW
    • Solid-state 561 nm – 100 mW
    • **Other lasers available upon request

Detector Parameters & Data Acquisition:

  • Forward scatter (FSC) – enables separation of unfixed platelets from noise
    • FSC resolution: < 500 nm polystyrene beads
    • FSC scales: log & linear
    • FSC: parameters: width, peak-height; area
    • Optional FSC photomultiplier (PMT) detector
  • Side scatter (SSC)- resolves lympho-, mono- , and granulocytes
    • SSC resolution: < 200 nm polystyrene beads
    • SSC scales: log & linear
    • SCC parameters: width, peak-height, area
  • Fluorescence channels:
    • Standard number of colors: 18
    • Sensitivity: < 100 MESF
    • PMT scales: log & linear
    • PMT parameters: width, peak-height, area
    • Time: 13 μsec resolution

Analysis Rate & Carryover

  • Analysis rate: 10,000 events/ sec
  • Carryover: <0.1% automatic backlash between samples

Sample Input:

  • Dead volume: < 8 μL
  • Minimum sample volume: < 20 μL
  • Maximum particle size: 40 μm
  • Patented low insertion force single tube loading

User Interchangeable Optical Filters:

  • Blue laser dyes: FITC (530/30), PE (580/30), PE- Texas Red (615/30), PerCP/PE-Cy5 (676/29), PECy5.5 (690/40), PE-Cy7 (740LP)
  • Red laser dyes: APC (676/29), APC-Cy5.5 (690/40), Alexa700 (710/40),  APC-Cy7 (740LP)
  • Green laser dyes: PE (580/30), PE- Texas Red (615/30), PerCP/PE-Cy5 (676/29), PECy5.5 (690/40), PE-Cy7 (740LP)
  • Violet laser dyes: Pacific Blue/DAPI (445/60), AmCyan (530/30), Pacific Orange (580/30), QD605 (615/30), QD655 (676/29), QD655 (676/29), QD705 (690/40), QD800 (740LP)
  • **Other dyes available upon request

Fluidics Tray:

  • Integrated fluidics tray does not increase the footprint of the instrument
  • Automated startup, shutdown, and cleaning cycles
  • Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
  • Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
  • Optional automated sheath tank fill and waste drainage

Operating Conditions:

  • Operating Conditions: 60-86 °F (15-30 °C)
  • Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
  • Weight (including fluidics tray): < 70 lbs (35 kg)
  • Power: 110/115/230 VAC, 50-60 Hz

 

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