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SE520EXi

Flow Cytometer

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We know that people demand performance and reliability from their flow cytometers, even for standard applications. But uncompromised performance simply would not be achievable at a lower price point with conventional entry-level systems. So we designed our SE520EXi flow cytometer with the same patented technologies as our S1000 series—without the same price tag.

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Features

  • Fixed configuration with 2 lasers (488nm and 640nm) and 6 colors
  • Based on the same performance and patented technology as the S1000EXi
  • Complete compatibility with Stratedigm’s full automation suite

 

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Technical Specifications

Laser:

  • System is designed to support up to 2 lasers with spatial separation
  • Laser options:
    • Solid-state 488 nm – 150 mW
    • Solid-state 640 nm – 100 mW

Detector Parameters & Data Acquisition:

  • Forward scatter (FSC) – enables separation of unfixed platelets from noise
    • FSC resolution: < 500 nm polystyrene beads
    • FSC scales: log & linear
    • FSC: parameters: width, peak-height; area
    • Optional FSC photomultiplier (PMT) detector
  • Side scatter (SSC)- resolves lympho-, mono- , and granulocytes
    • SSC resolution: < 200 nm polystyrene beads
    • SSC scales: log & linear
    • SCC parameters: width, peak-height, area
  • Fluorescence channels:
    • Standard number of colors: 6
    • Sensitivity: < 100 MESF
    • PMT scales: log & linear
    • PMT parameters: width, peak-height, area
    • Time: 13 µsec resolution

Analysis Rate & Carryover

  • Analysis rate: 10,000 events/ sec
  • Carryover: < 0.1% automatic backlash between samples

Sample Input:

  • Dead volume: < 8 μL
  • Minimum sample volume: < 20 μL
  • Maximum particle size: 40 μm
  • Patented low insertion force single tube loading

User Interchangeable Optical Filters:

  • Blue laser dyes: FITC (530/30), PE (580/30), PerCP/PE-Cy5 (676/29), PE-Cy7 (740LP)
  • Red laser dyes: APC (676/29), APC-Cy7 (740LP)

Fluidics Tray:

  • Integrated fluidics tray does not increase the footprint of the instrument
  • Automated startup, shutdown, and cleaning cycles
  • Automated decontamination procedure using onboard cleaning solution for all components in contact with sample
  • Tank Capacity: 4 L sheath, 4 L waste, 4 L auxiliary solution
  • Optional automated sheath tank fill and waste drainage

Operating Conditions:

  • Operating Conditions: 60-86 °F (15-30 °C)
  • Size (including fluidics tray): 21.5″ W x 21″ D x 24″ H (54.6 cm W x 53.3 cm D x 61 cm H)
  • Weight (including fluidics tray): < 70 lbs (35 kg)
  • Power: 110/115/230 VAC, 50-60 Hz

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